LOCUS       40924_45813        6021 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUT2 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Kasai Y, Oshima K, Ikeda F, Abe J, Yoshimitsu Y, Harayama S.
  TITLE     Construction of self-cloning system in a unicellular green algae, 
            Pseudochoricystis ellipsoidea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Kasai Y, Oshima K, Ikeda F, Abe J, Yoshimitsu Y, Harayama S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2014) Contact:Yuki Kasai Chuo University, 
            Biological Science; 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan
REFERENCE   3  (bases 1 to 6021)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6021)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-DEC-2014) Contact:Yuki Kasai Chuo University, Biological 
            Science; 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..790
                     /note="includes promoter region of Pseudochoricystis
                     ellipsoidea nuclear gene RBCS for ribulose bisphosphate 
                     carboxylase/oxygenase small subunit"
                     /regulatory_class="promoter"
     misc_feature    795..1073
                     /note="include intron 1 of Pseudochoricystis ellipsoidea
                     nuclear gene RBCS for ribulose bisphosphate 
                     carboxylase/oxygenase small subunit"
     CDS             1076..2578
                     /codon_start=1
                     /gene="UMPS"
                     /product="uridine monophosphate synthase"
                     /label=UMPS
                     /protein_id="BAR90702.1"
                     /translation="MATSTPSVATAAELKPKEGPITSQEFEELVLRLHEIEAVKFGNFK
                     LKSGLMSPIYIDLRVIVSYPDVLRRVAEVMWHQVSGAAFDVMCGVPYTALPIATCMSLL
                     HGTPMLMRRKEVKEYGTKKAIEGAFKKGQTCLIVEDLVTSGASVMETVEPLEVEGLKVT
                     DVVVLIDREQGGAARMASNGLRLHSAFTLSFIIKTLQAHGLVSAKVADSVAAFIAANQT
                     FTPSAAAPTPSPAAPAQPKRLPFEERASLCQNAAGRKLLELMARKRTNLAVAADVATVE
                     EMLRIADAAGPHIAVFKTHVDIFDKWDDGIATQLRHLADKHEFLIFEDRKFADIGNTVV
                     SQYGGGIYKIADWSDITNAHLVPGPGIIDGLRKVGQEKGRGLLLLAEMSSKGALATGAY
                     TEKVAEAAAANQDFVMGFICQSPAKWATPVPPGLVHMTPGVQLASGSDALGQQYNTPAS
                     VIGQGGSDVIIVGRGIIKAADPAAAAAQYREAGWAAYEATLA"
     gene            1076..2578
                     /gene="UMPS"
                     /label=UMPS
     regulatory      2594..3111
                     /note="includes terminator region of Pseudochoricystis 
                     ellipsoidea nuclear gene RBCS for ribulose bisphosphate 
                     carboxylase/oxygenase small subunit"
                     /regulatory_class="terminator"
     promoter        complement(3145..3163)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3184..3200)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3208..3224)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3232..3262)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3277..3298)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3586..4174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4348..5205)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5206..5310)
                     /label=AmpR promoter
     rep_origin      5337..5792
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5933..5949
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5959..5977
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgccctcaa tcggccgatt tcatgcatga agaagaagat gatgattaca catcattcca
       61 gccatctagg gctgccatca gtaattgcat gacccgcaac ttcccagtca gtcaaatgca
      121 caatagaagc atagttgcat gggaatcaac ccagcagcct cccgcagagc tagcctaggt
      181 acgtgtacgc agccacataa tcatcatgaa tgattctgca cggtgctatg atagcaacag
      241 atactcgcca gtgcgcacgc aagcaatctc atcacaccac tgtggtcagg caagcacgat
      301 atagatgtca aatgacagct ccattgggag tgcaagtcta ctagagtgca agcctactag
      361 tagtaggaag gtccagcgca tgccaaaact cctgctaagg ggttgcccaa gccccttggc
      421 tggccaaaag tccttaatat tttgctaggt aaaggggatc ttcccggagg tggtgttgat
      481 gaacatttgt acaccagcat ccgggtcctt ttggactctg aaaacgtgag cgagcagcga
      541 ctgcttgcag cttccaaacg aaaggtctgg aagcgctgga gtggtgagtc aggggtgact
      601 cagcaaacac caaggacaca cgttttcacc ctaccctcag aacccttgaa ataggggtcg
      661 atttccctct aacttaagtg gttatccaca aactcacgac gataagagcc agcgccagca
      721 ccatttcgtt cctcattttc agctgaaagc aaagcctttg catacttatc gctctcagat
      781 tacaatcgca atgggtacgc agctttctca ctccctcacc gcgtgtggtt cactcaggtt
      841 tctggctaag gaaagtctga tgtgactttc cttagacgtt aaagcagaat caccccatgc
      901 tttaagcagt tttatgtgtc ttgcccttca tgtgaagggt ctgtactgtt gcagagtgct
      961 ctcttgtgtt cttgagtgtt cttgtctcca gtgtgcccat taactttatc catgtcttat
     1021 gtgtccagga ctgcgccaaa gaaactgacg ctgcgctgtt ttgctttgtg cagccatggc
     1081 aacttcaact ccctcagtgg ccacagctgc ggagctgaaa ccaaaggaag ggcctatcac
     1141 gtcacaggag tttgaagagc tcgtgctgcg tttgcatgaa atcgaggctg tcaaattcgg
     1201 caacttcaag ctcaagagcg ggctcatgtc gccgatctac atcgacctgc gcgtcatcgt
     1261 gtcctatccg gacgtcctgc gcagggtggc ggaggtgatg tggcatcagg tgtcgggcgc
     1321 tgcatttgat gtgatgtgtg gcgtgccgta cacggcgctg cccatagcca catgcatgtc
     1381 gctgctgcac ggcacgccca tgctgatgcg gcgcaaggag gtcaaggagt acggcaccaa
     1441 gaaggccatt gagggcgcct tcaagaaggg ccagacctgc ctcattgtgg aggacctggt
     1501 caccagcggc gcctccgtca tggagactgt ggagcccctc gaggtggagg ggctgaaggt
     1561 gacggatgtg gtggtgctca tagaccggga gcagggcggc gcggcgcgca tggccagcaa
     1621 cgggctgcga ctgcactccg cgttcacgct ctccttcatc atcaagacgc tgcaggcgca
     1681 cggccttgtc agcgctaagg tggccgacag cgtcgctgcc ttcatagccg ccaaccagac
     1741 cttcaccccc tctgcagccg cccccacacc ctcacctgcc gctcctgcac agcccaaacg
     1801 gctgccattt gaggagcgcg caagcctatg tcagaacgca gccggcagga agctgttgga
     1861 gctgatggcg cgcaagcgga ccaacctggc ggtggcggcg gacgtggcga cggtggagga
     1921 gatgctacgc attgcggacg cggccgggcc acacatcgcc gtcttcaaga cgcacgtgga
     1981 catcttcgac aagtgggatg atggcatcgc cacccagctg cgccatctgg ctgacaagca
     2041 tgagttcttg atattcgagg acaggaagtt tgcggacatc ggtaacacag tggtgtcgca
     2101 gtacggcgga ggcatctaca agattgccga ctggtcagac atcaccaatg cacacctggt
     2161 ccctgggcca ggcatcatcg atggtctccg taaggtgggc caggagaagg ggcgcggcct
     2221 gctgttgctg gctgagatga gctccaaggg agcccttgcc acaggggcct acaccgagaa
     2281 ggttgctgaa gcggcagctg ccaaccagga ttttgtcatg ggcttcatct gccagtcacc
     2341 agccaagtgg gccacaccgg tccctccagg tctggtgcac atgacaccgg gagtgcagct
     2401 agcgtctggg agcgacgcat tggggcagca gtacaacaca cctgcttctg tcatcggcca
     2461 gggcggcagc gatgtcatca tcgtggggag gggaatcatt aaggcggcag accccgctgc
     2521 agcggccgca cagtacagag aagccggctg ggccgcctat gaggccacgc tggcatgaaa
     2581 gcttatcgat accgtcgaca ggttctaagc aatgacctgg gctgtacatc attagcaaac
     2641 tgaatctgca gctttctagg ctcttgctgt tcctaggaac ttggggttca agactgggtt
     2701 tttgctgtgg ttcctgactg cagaccatca ctgtctgggt tgtcgtgtct aatgtccata
     2761 ggtgtgagca catgctccac ttcaattcct actgctgggt aactggcgct gtctgaggga
     2821 cttctgagaa gaatgccgag aagaacttga gcgatttgta aggggatatt ttgggtctgt
     2881 accaaaggcc cagactgggc ttccagtgca tcgcacatat gcatctgcat gtccattcat
     2941 acaatcaacc tgttgactgc atgacccaga tcacaacctc cttgagttgg aagtagcttc
     3001 ttctacagat agaaaatggt gcatgtcagc tgagggttgc aggcgtttaa aagtgcatca
     3061 agtttgcaga acacatggtg attcatgact gcatggaaac agaccagcac actcgagggg
     3121 gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     3181 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3241 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     3301 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3361 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3421 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3481 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3541 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3601 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3661 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3721 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3781 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3841 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3901 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3961 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4021 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4081 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4141 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4201 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4261 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4321 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4381 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4441 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4501 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4561 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4621 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4681 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4741 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4801 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4861 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4921 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4981 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5041 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5101 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5161 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5221 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5281 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
     5341 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5401 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5461 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5521 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5581 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5641 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5701 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5761 gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt
     5821 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     5881 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     5941 acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct ccaccgcggt
     6001 ggcggccgct ctagaactag t
//
